Themes / Topics Wastewater/Sanitation
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March 23, 2025 In

Subsewershed analyses of the impacts of inflow and infiltration on viral pathogens and antibiotic resistance markers across a rural sewer system

Abstract/Summary: As wastewater-based surveillance (WBS) is increasingly used to track community-level disease trends, it is important to understand how pathogen signals can be altered by phenomena that occur within sewersheds such as inflow and infiltration (I&I). Our objectives were to characterize I&I across a rural sewershed and assess potential impacts on viral (rotavirus, norovirus GII, and SARS-CoV-2), fecal indicator (HF183, the hCYTB484 gene specific to the human mitochondrial genome, and crAssphage), and antimicrobial resistance (intI1, blaCTX−M-1) targets. In a small town in Virginia (USA), we collected 107 wastewater samples at monthly intervals over a 12-month period (2022–2023) at the wastewater treatment plant (WWTP) influent and 11 up-sewer sites. Viral, fecal indicator, and antimicrobial resistance targets were enumerated using ddPCR. We observed the highest concentrations of human fecal markers and a measure anthropogenic pollution and antibiotic resistance (intI1) in up-sewer sites with limited I&I. However, median viral gene copy concentrations were highest at the WWTP, compared to 100 % (n = 11), 90 % (n = 10), and 55 % (n = 6) of up-sewer sites for rotavirus, norovirus GII, and SARS-CoV-2, respectively. After adjusting for covariates (Ba, COD, dissolved oxygen, groundwater depth, precipitation, sampling date) using generalized linear models, moderate to high I&I was associated with statistically significant reductions in log10-transformed rotavirus and norovirus GII concentrations across the sewershed (coefficients = -0.7 and -0.9, p < 0.001, n = 95), though not for SARS-CoV-2 (coefficient = -0.2, p = 0.181, n = 95). Overall, we found that while I&I can diminish biomarker signals throughout a sewershed, including at the WWTP influent, I&I impacts vary depending on the target, and pathogen biomarker signals were, on average, higher and less variable over time at the WWTP compared to most up-sewer sites. As far as we are aware, this is the first study to assess in situ I&I impacts on multiple WBS targets. Taken together, our findings highlight challenges and tradeoffs associated with different sampling strategies for different WBS targets in heavily I&I impacted systems.

March 23, 2025 In

Drinking water sources, quality, and associated health outcomes in Appalachian Virginia: A risk characterization study in two counties

Abstract/Summary: In the US, violations of drinking water regulations are highest in lower-income rural areas overall, and particularly in Central Appalachia. However, data on drinking water use, quality, and associated health outcomes in rural Appalachia are limited. We sought to assess public and private drinking water sources and associated risk factors for waterborne pathogen exposures for individuals living in rural regions of Appalachian Virginia. We administered surveys and collected tap water, bottled water, and saliva samples in lower-income households in two adjacent rural counties in southwest Virginia (bordering Kentucky and Tennessee). Water samples were tested for pH, temperature, conductivity, total coliforms, E. coli, free chlorine, nitrate, fluoride, heavy metals, and specific pathogen targets. Saliva samples were analyzed for antibody responses to potentially waterborne infections. We also shared water analysis results with households. We enrolled 33 households (83 individuals), 82% (n = 27) with utility-supplied water and 18% with private wells (n = 3) or springs (n = 3). 58% (n = 19) reported household incomes of <$20,000/year. Total coliforms were detected in water samples from 33% (n = 11) of homes, E. coli in 12%, all with wells or springs (n = 4), and Aeromonas, Campylobacter, and Enterobacter in 9%, all spring water (n = 3). Diarrhea was reported for 10% of individuals (n = 8), but was not associated with E. coli detection. 34% (n = 15) of saliva samples had detectable antibody responses for Cryptosporidium spp., C. jejuni, and Hepatitis E. After controlling for covariates and clustering, individuals in households with septic systems and straight pipes had significantly higher likelihoods of antibody detection (risk ratios = 3.28, 95%CI = 1.01–10.65). To our knowledge, this is the first study to collect and analyze drinking water samples, saliva samples, and reported health outcome data from low-income households in Central Appalachia. Our findings indicate that utility-supplied water in this region was generally safe, and individuals in low-income households without utility-supplied water or sewerage have higher exposures to waterborne pathogens.

January 2, 2024 In

Making Waves: The Benefits and Challenges of Responsibly Implementing Wastewater-based Surveillance for Rural Communities

Abstract/Summary: The sampling and analysis of sewage for pathogens and other biomarkers offers a powerful tool for monitoring and understanding community health trends and potentially predicting disease outbreaks. Since the early months of the COVID-19 pandemic, the use of wastewater-based testing for public health surveillance has increased markedly. However, these efforts have focused on urban and peri-urban areas. The potential public health benefits of wastewater-based surveillance for rural communities are substantial – though so too are the methodological and ethical challenges. Practitioner observations and research findings indicate that for many rural systems, typical implementation approaches for wastewater-based surveillance will not yield sufficiently reliable or actionable results. In this paper, we discuss key challenges and potential strategies to address them. However, to support and expand the implementation of responsible, reliable, and ethical wastewater-based surveillance for rural communities, best practice guidelines and standards are needed.

January 2, 2023 In

Drinking Water & Health in Rural Appalachia

We are working on a number of projects to better understand issues related to drinking water (access, quality, use), sanitation, and reported and measured health outcomes in the Appalachia region, with a focus on children and adults living in Central Appalachia. In 2020, we started a comprehensive systematic review and meta-analysis study to identify and synthesize published research on drinking water contamination and associated health outcomes in the Appalachian region over a 20-year time period. We pre-registered our study protocols, completed data extraction and analysis in 2022, and published our findings in 2023 – the limited number and nature of the studies identified demonstrated that more epidemiologic research is needed to understand exposures to drinking water contaminants in this region. From 2021 to 2023, in collaboration with UVA, ETSU, a regional non-profit, a local utility, and others, we conducted a water-and-health focused cross-sectional study and a prospective cohort study in southwest VA (an important component of these studies is that we also share water testing results with participating households). In 2022, we published findings from the cross-sectional study (a small community of households with private wells), and we anticipate publishing preliminary findings from the cohort study (rural households with utility-supplied and private well and spring water in two counties) in early 2024. Given the relatively high rates of bottled water use/reliance we observed in rural households, we also conducted a study to analyze the quality of bottled water sold in the region; we anticipate publishing those findings in 2024. Building on findings from these studies, in late 2023 (in collaboration with ETSU, a regional non-profit, and others) we initiated a prospective safe water intervention pilot study in rural regions of southwest VA and northeast TN.

July 21, 2022 In

Subsewershed SARS-CoV-2 Wastewater Surveillance & COVID-19 Epidemiology Using Building-specific Occupancy & Case Data

Abstract/Summary: To evaluate the use of wastewater-based surveillance and epidemiology to monitor and predict SARS-CoV-2 virus trends, over the 2020–2021 academic year we collected wastewater samples twice weekly from 17 manholes across Virginia Tech’s main campus. We used data from external door swipe card readers and student isolation/quarantine status to estimate building-specific occupancy and COVID-19 case counts at a daily resolution. After analyzing 673 wastewater samples using reverse transcription quantitative polymerase chain reaction (RT-qPCR), we reanalyzed 329 samples from isolation and nonisolation dormitories and the campus sewage outflow using reverse transcription digital droplet polymerase chain reaction (RT-ddPCR). Population-adjusted viral copy means from isolation dormitory wastewater were 48% and 66% higher than unadjusted viral copy means for N and E genes (1846/100 mL to 2733/100 mL/100 people and 2312/100 mL to 3828/100 mL/100 people, respectively; n = 46). Prespecified analyses with random-effects Poisson regression and dormitory/cluster-robust standard errors showed that the detection of N and E genes were associated with increases of 85% and 99% in the likelihood of COVID-19 cases 8 days later (incident–rate ratio (IRR) = 1.845, p = 0.013 and IRR = 1.994, p = 0.007, respectively; n = 215), and one-log increases in swipe card normalized viral copies (copies/100 mL/100 people) for N and E were associated with increases of 21% and 27% in the likelihood of observing COVID-19 cases 8 days following sample collection (IRR = 1.206, p < 0.001, n = 211 for N; IRR = 1.265, p < 0.001, n = 211 for E). One-log increases in swipe normalized copies were also associated with 40% and 43% increases in the likelihood of observing COVID-19 cases 5 days after sample collection (IRR = 1.403, p = 0.002, n = 212 for N; IRR = 1.426, p < 0.001, n = 212 for E). Our findings highlight the use of building-specific occupancy data and add to the evidence for the potential of wastewater-based epidemiology to predict COVID-19 trends at subsewershed scales.

October 18, 2021 In

Poverty, Water, Climate Change, & Health in Sub-Saharan Africa

As described below (see other project summaries), the Multidimensional Poverty Assessment Tool (MPAT) was created in two phases (from 2008 to 2014) via a collaborative, international initiative to develop, test, and pilot a new tool for local-level rural poverty assessment. The work was guided by a Sounding Board of experts from the International Fund for Agricultural Development (IFAD), other United Nations agencies, international and regional organizations, and universities around the world (see www.ifad.org/mpat). Following MPAT’s finalization and institutionalization in 2014, it was used in multiple countries including many in Sub-Saharan Africa. We are currently working on multiple desk-based studies with a variety of collaborators to analyze data from ~7,000 households across Eswatini, Kenya, Lesotho, Mali, Tanzania, and Zimbabwe. Because MPAT was developed based primarily on data from Bangladesh, China, India, and Mozambique, one study we are leading is an updated assessment and evaluation of MPAT’s indicator structure and robustness based on its use in the sub-Saharan region. Other studies we are working on focus on a cross-county analyses of MPAT’s water, climate change, and health focused components and sub-components, and related programs and interventions. We anticipate publishing a number of papers on this research in 2024.

October 18, 2021 In

Wastewater Surveillance & Epidemiology at Multiple Scales

As of 2022, our research efforts have primarily focused on wastewater-based surveillance (WBS) and epidemiology in rural settings, with the overarching goal of developing improved methods and guidelines for the implementation of responsible, reliable, and ethical WBS for rural communities. The foundation of this effort was established during the 2020/21 academic year, when Dr.s Pruden & Vikesland in Environmental Engineering led an initiative with collaborators across Virginia Tech (and at HRSD) to collect and analyze wastewater samples from different buildings and sites across the university campus with the goal of developing a coronavirus disease-2019 (COVID-19) surveillance program. WBS has been used by many other research groups, as well as wastewater utilities, to monitor SARS-CoV-2 (i.e., the virus that causes COVID-19) trends. Our primary research objective for that study was to evaluate the use of WBS and epidemiologic methods to monitor and predict SARS-CoV-2 virus trends at sub-sewershed scales. Using pre-specified analyses, we showed that the detection of SARS-CoV-2 genes in wastewater samples was associated with statistically significant increases in COVID-19 cases 8 days after sample collection. We published our methods and findings from this research in 2022. In the summer of 2022, our research group – in collaboration with VT faculty members Pruden, Vikesland, Krometis, students in their groups, and Dr. Taniuchi’s group at UVA – initiated a WBS research study in partnership with a utility in southwest Virginia. We started a monthly sub-sewershed sampling campaign in September 2022 (photo is of a composite sampler at the wastewater treatment plant), which was completed in August 2023. We anticipate publishing initial findings from this research in 2024, and are currently working to build and expand on these efforts at other sites in 2024 and 2025, in collaboration with the Virginia Department of Health and others.

March 1, 2021 In

Refinement & Finalization of the Multidimensional Poverty Assessment Tool (MPAT)

Following the release of the working-paper User’s Guide for the Multidimensional Poverty Assessment Tool (MPAT) in 2009, a number of agencies and universities used the beta-version of MPAT in a variety of settings. In order to finalize MPAT and develop a comprehensive User’s Guide and associated resources, we built on the lessons learned from early adopters of the the tool (e.g., an NGO in Kenya) and iteratively used and evaluated the tool with IFAD-supported projects in Bangladesh and Mozambique. Details on the participatory expert elicitation methods we used are provided in a Journal of Development Studies paper. We developed an Excel-based data entry platform so users could easily calculate MPAT’s indicators at household, village, and project levels. We also wrote a comprehensive, 300+ page, 2014 MPAT User’s Guide which provides step-by-step instructions for using MPAT as well as training modules and materials, all with the goal of making MPAT an accessible open-source tool. The User’s Guide and accompanying resources were presented at a 2014 launch event in Rome. Since its 2014 release, MPAT has been translated into a number of languages, an optional 11th component focused on climate change was added, and MPAT has been used by a variety of agencies and institutions around the world. MPAT publications and related resources are available at www.ifad.org/mpat.

March 1, 2021 In

Wastewater & Coastal Environmental Health in the Philippines

This research collaboration between colleagues at the University of California Berkeley (UCB) and the University of the Philippines (UPD) was designed to advance methods for measuring, modelling, and assessing marine water quality and environmental health at three sites in the Philippines. One of the three study sites for this multi-year project is Boracay Island. A popular tourist destination, in recent years sewage and other contaminants have adversely impacted Boracay’s beaches and coastal water quality, with implications for both environmental and human health. This research project centers on the use of an autonomous surface vessel to collect a wide range of spatially referenced data which can be combined with data from water samples and lab-based analyses, as well as satellite and other remote sensed data. At the Boracay site, proxy sensor data (e.g., optical brighteners and tryptophan) and laboratory analyses of grab samples will be combined to map and model actual and estimated water quality and environmental health impacts from point and non-point runoff and wastewater effluent. Our planned research activities and associated timelines suffered from a variety of logistical setbacks and delays, as well as delays related to the COVID-19 Pandemic. We anticipate publishing findings from this work starting in 2023.

December 31, 2020 In

Developing a Thematic Indicator for Rural Poverty Assessment (MPAT)

The Multidimensional Poverty Assessment Project was a collaborative, international initiative to develop, test, and pilot a new tool for local-level rural poverty assessment. The work was supported by IFAD and guided by a Sounding Board of experts from IFAD, other United Nations agencies, international and regional organizations, and universities around the world, with the majority of its members coming from the Asia region where we developed and tested the tool in China and India. Surveys (household and village level) were developed and tested in an iterative and participatory fashion, as was the indicator structure used to aggregate sub-components and components. The resulting Multidimensional Poverty Assessment Tool (MPAT) provides an assessment, an overview, of ten dimensions central to rural livelihoods, highlighting where additional support or interventions are likely to be most needed. The tool was designed to be universal enough to be relevant to most rural contexts around the world, yet specific enough to provide project managers and others a detailed overview of key dimensions relevant to rural poverty reduction efforts. MPAT was independently evaluated by the European Commission Joint Research Center and a working-paper version of MPAT User’s Guide was released in 2009 as well as the 2009 MPAT Book, which provides a detailed description of why and how the tool was developed. MPAT’s theoretical foundations are described in a 2010 article in Development and Practice.